3FAS
X-ray structure of iGluR4 flip ligand-binding core (S1S2) in complex with (S)-glutamate at 1.40A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-06-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0412 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.425, 105.231, 66.538 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 26.600 - 1.400 |
| Rwork | 0.167 |
| R-free | 0.18300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FTJ (poly-Ala) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.226 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.778 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.092 | 0.405 |
| Total number of observations | 58435 | |
| Number of reflections | 124382 | |
| <I/σ(I)> | 11.3 | 2.3 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 280 | PEG4000, Acetate, (NH4)2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
