3FAL
humanRXR alpha & mouse LXR alpha complexed with Retenoic acid and GSK2186
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.212, 90.002, 101.311 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 42.342 - 2.360 |
| R-factor | 0.2223 |
| Rwork | 0.219 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.342 | |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 41753 | |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 3uL 6.6mgs/ml protein + 3 uL well containing 21 % - 23 % PEG 3550, 200mM Ammonium Acetate, 100 mM Bis Tris pH 6.5 and 10 mM DTT, VAPOR DIFFUSION, SITTING DROP |
