3F7R
First pair of Fibronectin type III domains and part of the connecting segment of the integrin beta4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.500, 42.150, 56.910 |
| Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
| Resolution | 35.674 - 2.036 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qg3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.674 | 2.110 |
| High resolution limit [Å] | 2.036 | 2.036 |
| Number of reflections | 19563 | |
| <I/σ(I)> | 16.6 | 5.6 |
| Completeness [%] | 98.2 | 85.9 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M Cacodylate, 18.75% PEG 600, 6.25% Glycerol, 0.7M NaCl, pH 6.5, VAPOR DIFFUSION, temperature 298K |
