3F6Q
Crystal structure of integrin-linked kinase ankyrin repeat domain in complex with PINCH1 LIM1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.004, 41.500, 46.469 |
| Unit cell angles | 78.01, 68.98, 85.91 |
Refinement procedure
| Resolution | 19.890 - 1.600 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19900 |
| Starting model (for MR) | 2bkg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 33486 | |
| <I/σ(I)> | 13 | 2.6 |
| Completeness [%] | 92.5 | 87.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 296 | 20% PEG 3350, 0.2M Sodium formate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
