3F5P
Complex Structure of Insulin-like Growth Factor Receptor and 3-Cyanoquinoline Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 143 |
| Detector technology | CCD |
| Collection date | 2004-01-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.340, 137.020, 178.990 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 19.986 - 2.900 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zm3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.095 | 0.434 |
| Number of reflections | 133523 | |
| <I/σ(I)> | 2.67 | |
| Completeness [%] | 97.0 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris-HCl, pH8.5, 2M NaCl, 14% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
