3F4Y
HIV gp41 six-helix bundle containing a mutant CHR alpha-peptide sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-05 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 37.611, 179.038, 33.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.26000 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.146 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.800 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 15938 | |
<I/σ(I)> | 28 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 1 M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 298K |