3F4N
Crystal Structure of Pyridoxal Phosphate Biosynthetic Protein PdxJ from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.627, 114.818, 154.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.810 - 2.402 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1N5W |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.810 | 2.440 |
| High resolution limit [Å] | 2.402 | 2.402 |
| Rmerge | 0.108 | 0.600 |
| Number of reflections | 75718 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 95.6 | 88.7 |
| Redundancy | 6.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.2 M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3,350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
