3EZZ
Crystal Structure of human MKP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2005-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 |
| Unit cell lengths | 141.557, 141.557, 158.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.24200 |
| Starting model (for MR) | 2g6z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.071 | 0.386 |
| Number of reflections | 34516 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.2 | 291 | 0.1M CAPS pH 10.2, 0.1M NaCl, 1.7M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
