3EZS

Crystal structure of aminotransferase AspB (NP_207418.1) from HELICOBACTER PYLORI 26695 at 2.19 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	92.648, 106.249, 80.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.281 - 2.190
R-factor	0.161
Rwork	0.159
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.281	44.281	2.270
High resolution limit [Å]	2.190	4.700	2.190
Rmerge	0.112	0.041	0.612
Number of reflections	40833	4373	3568
<I/σ(I)>	12.8	32.4	2.29
Completeness [%]	98.3	98.1	85.8
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	15.0000% Glycerol, 8.5000% iso-Propanol, 17.0000% PEG-4000, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K