3EZ5
Cocrystal structure of Bacillus fragment DNA polymerase I with duplex DNA , dCTP, and zinc (closed form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.282420 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.551, 108.572, 149.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.830 - 1.900 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.24400 |
| Starting model (for MR) | 2hvi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.382 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.398 |
| Number of reflections | 119138 | 32718 |
| <I/σ(I)> | 12.28 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.78 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | Ammonium Sulfate, MES, MPD, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | Ammonium Sulfate | ||
| 5 | 1 | 2 | MSE | ||
| 6 | 1 | 2 | MPD |
