3EY6
Crystal structure of the FK506-binding domain of human FKBP38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-06-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.057, 60.288, 30.840 |
| Unit cell angles | 90.00, 97.59, 90.00 |
Refinement procedure
| Resolution | 30.500 - 1.050 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2awg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.038 | 0.114 |
| Total number of observations | 7827 | |
| Number of reflections | 44414 | |
| <I/σ(I)> | 11.709 | 5.9 |
| Completeness [%] | 90.4 | 52.3 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30% PEG 4000, 0.2M calcium chloride, 0.1M TRIS, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
