3EXS

Crystal structure of KGPDC from Streptococcus mutans in complex with D-R5P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-21
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	59.152, 124.249, 130.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.740 - 2.500
R-factor	0.18661
Rwork	0.184
R-free	0.24386
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ext
RMSD bond length	0.013
RMSD bond angle	1.367
Data scaling software	SAINT
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	19.740
High resolution limit [Å]	2.500
Number of reflections	31798
Completeness [%]	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.2M KCl, 0.05M HEPES, pH7.5, 35%(v/v) pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 289K