3EW3

the 1:2 complex between a Nterminal elongated prolactin and the extra cellular domain of the rat prolactin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	77
Detector technology	CCD
Collection date	2006-12-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.975
Spacegroup name	P 43 21 2
Unit cell lengths	92.120, 92.120, 215.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.997 - 3.800
R-factor	0.256
Rwork	0.252
R-free	0.32370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f6f
RMSD bond length	0.009
RMSD bond angle	1.247
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.21)
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	107.833	107.830	3.900
High resolution limit [Å]	3.500	11.700	3.700
Rmerge	0.093	0.067	0.176
Total number of observations		1361	7639
Number of reflections	10464
<I/σ(I)>	6.536	9.5	3.9
Completeness [%]	99.2	94.5	99.9
Redundancy	4.9	3.5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	100mM LiSO4, 100mM NaCitrate pH 5.6, 12% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K