3EVI
Crystal structure of the thioredoxin-fold domain of human phosducin-like protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 44.594, 44.594, 141.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.880 - 2.700 |
| R-factor | 0.23615 |
| Rwork | 0.234 |
| R-free | 0.27576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dbc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.140 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.690 |
| Rmerge | 0.175 | 0.614 |
| Number of reflections | 8421 | |
| <I/σ(I)> | 10.22 | 1.53 |
| Completeness [%] | 93.9 | 88.6 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 28%(w/v) PEG8000, 0.2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
