3EUE
Crystal structure of ligand-free human uridine phosphorylase 1 (hUPP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 253.777, 253.777, 253.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.20468 |
| Rwork | 0.204 |
| R-free | 0.22099 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1c |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.675 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.384 |
| Number of reflections | 31733 | |
| <I/σ(I)> | 30.7 | 5.72 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 1.2M Ammonium sulfate, 1% MPD, 100 mM Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
