3ETM
Crystal structure of the mimivirus NDK +KPN-N62L-R107G triple mutant complexed with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-09-27 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.325, 70.325, 104.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.231 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22776 | |
| <I/σ(I)> | 6.9 | 2.2 |
| Completeness [%] | 98.9 | 94.5 |
| Redundancy | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.8 to 1M sodium citrate, 0.1M Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
