3EPS
The crystal structure of isocitrate dehydrogenase kinase/phosphatase from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.919 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.595, 124.595, 267.635 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.26800 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.097 | |
Number of reflections | 45600 | |
<I/σ(I)> | 305.7 | 1.4 |
Completeness [%] | 86.5 | 96.6 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12% PEG8000, 0.2M magnesium chloride, 0.1M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |