3ENA
Crystal structure of the mimivirus NDK +Kpn-N62L-R107G triple mutant complexed with dGDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-09-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 63 |
| Unit cell lengths | 70.358, 70.358, 104.295 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.611 - 1.600 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.191 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.600 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.042 | 0.229 |
| Number of reflections | 38444 | |
| <I/σ(I)> | 11.2 | 3.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.8 to 1.1M sodium citrate, 0.1M tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
