3EMB
Wesselsbron virus Methyltransferase in complex with AdoMet and 7MeGpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.236, 61.297, 128.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.030 - 2.300 |
| R-factor | 0.16921 |
| Rwork | 0.166 |
| R-free | 0.22884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3elu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.549 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.020 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.137 | 0.539 |
| Number of reflections | 13695 | |
| <I/σ(I)> | 11.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10mM AdoMet, 1mM 7MeGpppG, 18% PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
