3EL7
Crystal structure of c-Src in complex with pyrazolopyrimidine 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.360, 63.130, 56.050 |
| Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.2201 |
| Rwork | 0.220 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yoj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.331 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 7350 | |
| <I/σ(I)> | 11.97 | 4.55 |
| Completeness [%] | 99.4 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 287 | 100mM NaCl, 10mM Tris-HCl, 5% Glycerol, 2mM DTT, 4% DMSO, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
