3EJJ
Structure of M-CSF bound to the first three domains of FMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9766 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.848, 158.848, 237.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.241 |
| Rwork | 0.238 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hmc |
| RMSD bond angle | 0.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.042 | 0.467 |
| Number of reflections | 44786 | |
| <I/σ(I)> | 17.9 | 2.5 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 295 | 12% PEG 3000, 0.1M Hepes, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
