3EJ8

Structure of double mutant of human iNOS oxygenase domain with bound immidazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	89.883, 150.710, 191.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.850 - 2.550
Rwork	0.232
R-free	0.27100
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.850
High resolution limit [Å]	2.550
Number of reflections	77427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K