3EGV

Ribosomal protein L11 methyltransferase (PrmA) in complex with trimethylated ribosomal protein L11

Replaces:  3CJU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9797
Spacegroup name	P 62
Unit cell lengths	134.547, 134.547, 48.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.18416
Rwork	0.183
R-free	0.21332
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 2NXC 2nxn
RMSD bond length	0.015
RMSD bond angle	1.503
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.056	0.334
Number of reflections	48051
<I/σ(I)>	12.2	2
Completeness [%]	97.2	93.8
Redundancy	1.7	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch technique under oil	6.5	277	160 mM calcium acetate hydrate, 80 mM sodium cacodylate, 14.4% w/v PEG8000, 20% v/v glycerol, 4mM AdoMet, pH 6.5, microbatch technique under oil, temperature 277.0K