3EFH
Crystal structure of human phosphoribosyl pyrophosphate synthetase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | H 3 |
| Unit cell lengths | 169.180, 169.180, 61.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.21649 |
| Rwork | 0.216 |
| R-free | 0.23133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hcr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.295 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.065 | 0.345 |
| Number of reflections | 18518 | |
| <I/σ(I)> | 8.5 | 1.8 |
| Completeness [%] | 91.2 | 88.5 |
| Redundancy | 1.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 295 | 2.1M ammonium sulfate, 0.1M sodium acetate, 10% (v/v) glycerol, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
