3EF7

ZP-N domain of mammalian sperm receptor ZP3 (crystal form III)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.28235
Spacegroup name	P 21 21 2
Unit cell lengths	86.200, 98.580, 143.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.160 - 3.100
R-factor	0.21
Rwork	0.208
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT AND ZN-SINGLE-WAVELENGTH ANOMALOUS DIFFRACTION
Starting model (for MR)	3d4g
RMSD bond length	0.011
RMSD bond angle	1.039
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	3.170
High resolution limit [Å]	3.100	3.100
Number of reflections	42898
<I/σ(I)>	17.7	3.1
Completeness [%]	99.9	100
Redundancy	7.6	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	Sample: 15mg/ml protein in 0.05M sodium chloride, 0.01M Tris-HCL, PH7.2, 0.001M Maltose. Reservoir: 12% PEG6000, 0.15M calcium chloride, 0.0025M zinc chloride, 0.1M Tris-HCL, PH8.2. Sample to reservoir ratio in drop: 1:1, PH8.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K, pH7.8