3EF5

Structure of the RNA pyrophosphohydrolase BdRppH in complex with dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-16
Detector	adsc Q210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	68.446, 68.448, 92.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.370 - 2.600
R-factor	0.243
Rwork	0.240
R-free	0.29700
RMSD bond length	0.010
RMSD bond angle	1.246
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.370	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.049	0.027	0.691
Number of reflections	15730
<I/σ(I)>	32.267
Completeness [%]	89.2	99.4	40.7
Redundancy	6.6	6.7	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	298	PEG 4000, Na acetate, pH 7, hanging drop, temperature 298K