3EE6
Crystal Structure Analysis of Tripeptidyl peptidase -I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.450, 128.930, 100.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.210 - 2.350 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | SADABS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.034 | 0.312 |
| Number of reflections | 61321 | |
| <I/σ(I)> | 17.2 | 3.15 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 7.18 | 6.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.9 | 293 | 7% PEG 4000, 0.02M zinc sulfate, 0.1M sodium acetate, 0.1M ammonium sulfate, pH 4.9, VAPOR DIFFUSION, temperature 293K |
