3EDY
Crystal Structure of the Precursor Form of Human Tripeptidyl-Peptidase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.807, 93.173, 102.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.720 - 1.850 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.20500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.079 | 0.457 |
| Number of reflections | 50890 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.1 | 93.1 |
| Redundancy | 7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 278 | PEG 6000, citrate, pH 5.0, vapor diffusion, hanging drop, temperature 278K |
