3EDH
Crystal structure of bone morphogenetic protein 1 protease domain in complex with partially bound DMSO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.499, 59.093, 69.451 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.990 - 1.250 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3edi |
RMSD bond length | 0.013 |
RMSD bond angle | 1.578 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.090 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.083 | 0.394 |
Number of reflections | 60273 | |
<I/σ(I)> | 15.5 | 3.66 |
Completeness [%] | 97.9 | 96.1 |
Redundancy | 6 | 5.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 5% DMSO, pH 7.5, vapor diffusion, temperature 277K |