3EBO
Glycogen Phosphorylase b/Chrysin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-06-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.038 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.834, 128.834, 116.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.230 - 1.900 |
| R-factor | 0.19573 |
| Rwork | 0.194 |
| R-free | 0.21957 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 71586 | |
| <I/σ(I)> | 15.6 | 4.4 |
| Completeness [%] | 97.3 | 96.7 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 286 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 286K |
