3EB4
Voltage-dependent K+ channel beta subunit (I211R) in complex with cortisone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97893 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 113.260, 113.260, 147.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.040 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exb chain A beta subunit |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.064 | 0.460 |
| Number of reflections | 35198 | |
| <I/σ(I)> | 35.66 | 4.48 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6-15% glycerol, 1.5 M ammonium sulfate, 0.1 M Tris, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
