3EAK
NbBCII10 humanized (FGLA mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.909 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.585, 70.750, 50.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| Rwork | 0.215 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zmy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.370 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.196 | 0.607 |
| Number of reflections | 20171 | |
| <I/σ(I)> | 9.99 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM Na-Hepes pH 7.5, 2% PEG 400, 2M NH4(SO4)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
