3E9V
Crystal structure of human B-cell Translocation Gene 2 (BTG2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.535, 41.023, 68.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.220 - 1.700 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d5r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.030 | 41.030 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.071 | 0.046 | 0.457 |
| Total number of observations | 5388 | 23988 | |
| Number of reflections | 12646 | ||
| <I/σ(I)> | 6.69 | 12.7 | 1.5 |
| Completeness [%] | 98.7 | 97.3 | 97.6 |
| Redundancy | 13.8 | 11.7 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 0.2M Magnesium Chloride, 25% PEG 3350, Vapor diffusion, sitting drop, temperature 294K |
