3E80

Structure of Heparinase II complexed with heparan sulfate degradation disaccharide product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	201.280, 209.360, 59.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.570 - 2.350
R-factor	0.231
Rwork	0.229
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fuq
RMSD bond length	0.010
RMSD bond angle	1.554
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.570	37.570	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.134	0.059	0.302
Total number of observations		51933	35822
Number of reflections	103320
<I/σ(I)>	8.3	15.9	4.4
Completeness [%]	98.1	98.1	90
Redundancy	4.62	4.72	3.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1