3E7J
HeparinaseII H202A/Y257A double mutant complexed with a heparan sulfate tetrasaccharide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.951, 162.210, 93.838 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 42.520 - 2.100 |
| R-factor | 0.20116 |
| Rwork | 0.199 |
| R-free | 0.23450 |
| Starting model (for MR) | 2fut |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.966 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.429 |
| Number of reflections | 72010 | |
| <I/σ(I)> | 15.5 | 1.5 |
| Completeness [%] | 82.9 | 36.1 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
