3E4Z
Crystal structure of human insulin degrading enzyme in complex with insulin-like growth factor II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 65 |
| Unit cell lengths | 263.033, 263.033, 90.816 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.280 |
| R-factor | 0.19746 |
| Rwork | 0.196 |
| R-free | 0.22983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.336 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.134 | 0.507 |
| Number of reflections | 162414 | |
| <I/σ(I)> | 20.1 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10-13% PEG MME 5000, 100 mM HEPES pH 7.0, 4-14% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
