3E1Y

Crystal structure of human eRF1/eRF3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Wavelength(s)	0.9793
Spacegroup name	P 43
Unit cell lengths	173.966, 173.966, 119.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.800
R-factor	0.26193
Rwork	0.260
R-free	0.30358
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dt9 1r5b
RMSD bond length	0.009
RMSD bond angle	1.469
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.800
Rmerge	0.068
Number of reflections	35459
<I/σ(I)>	11.5
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	288	50mM MES, pH5.6, 20-22% ethylene glycol, 2mM DTT, 2mM ATP, EVAPORATION, temperature 288K