3E0D

Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77
Detector technology	CCD
Collection date	2007-03-09
Wavelength(s)	1.000
Spacegroup name	P 41
Unit cell lengths	149.546, 149.546, 163.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 4.600
Rwork	0.287
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	4.600	4.600
Number of reflections	20097

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	16 - 21% PEG 400, 100 mM CaCl2, 100 mM HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K
1	VAPOR DIFFUSION, SITTING DROP		289.15	16 - 21% PEG 400, 100 mM CaCl2, 100 mM HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 289.15K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	CaCl2
3	1	1	HEPES
4	1	1	H2O
5	1	2	PEG 400
6	1	2	CaCl2
7	1	2	HEPES
8	1	2	H2O