3DYQ
human phosphodiestrase 9 (inhibited by omitting divalent cation) in complex with cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.650, 103.650, 270.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.19885 |
Rwork | 0.196 |
R-free | 0.22627 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1TBM |
RMSD bond length | 0.014 |
RMSD bond angle | 1.350 |
Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.085 |
Number of reflections | 65299 |
<I/σ(I)> | 23 |
Completeness [%] | 98.7 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |