3DYD
Human Tyrosine Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.420, 91.590, 75.570 |
| Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
| Resolution | 36.910 - 2.300 |
| R-factor | 0.22158 |
| Rwork | 0.219 |
| R-free | 0.26088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.115 | 0.492 |
| Number of reflections | 44821 | |
| <I/σ(I)> | 10.7 | 3.3 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% MPD, 0.1M Na-Acetate pH 4.6, 3mM PLP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
