3DY8

Human Phosphodiesterase 9 in complex with product 5'-GMP (E+P complex)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	103.394, 103.394, 269.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.150
R-factor	0.17707
Rwork	0.175
R-free	0.19879
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.016
RMSD bond angle	1.444
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.230
High resolution limit [Å]	2.150
Rmerge	0.058
Number of reflections	77975
Completeness [%]	96.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1