3DX3
Golgi alpha-Mannosidase II in complex with Mannostatin analog (1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.062, 109.977, 138.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.420 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hty |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.470 |
| Data reduction software | HKL-2000 |
| Data scaling software | SADABS |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.074 | 0.260 |
| Number of reflections | 193233 | |
| <I/σ(I)> | 18.2 | 6.6 |
| Completeness [%] | 97.1 | 78.8 |
| Redundancy | 7.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG8000, Tris, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7 |
