3DWF

Crystal Structure of the Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1 Mutant F278E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	78.080, 85.920, 176.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.680 - 2.200
Rwork	0.200
R-free	0.25500
RMSD bond length	0.005
RMSD bond angle	0.923
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	34.680
High resolution limit [Å]	2.200
Number of reflections	60967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	291	0.1M Tris-HCl, 1.75M ammonium sulphate and 5% glycerol, pH 8, Vapor diffusion, temperature 291K