3DUH
Structure of Interleukin-23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Collection date | 2008-05-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.089, 59.974, 160.365 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.23007 |
| Rwork | 0.228 |
| R-free | 0.26833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human p40 from PDB ID 1F45 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.527 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.556 |
| Number of reflections | 49424 | |
| <I/σ(I)> | 13.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 20% PEG3350, 0.2M potassium nitrate, 0.1M Hepes-NaOH pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
