3DRA
Candida albicans protein geranylgeranyltransferase-I complexed with GGPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.304, 66.049, 82.821 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 37.640 - 1.800 |
| R-factor | 0.19398 |
| Rwork | 0.192 |
| R-free | 0.23056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 63895 | |
| Completeness [%] | 98.2 | 76.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | PEG 1500, propionate-cacodylate-bis-tris buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
