3DM7

Crystal Structure of the Vps75 Histone Chaperone

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9789,0.9791,0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.010, 89.658, 94.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.209
R-free	0.24000
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.070
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	45182
<I/σ(I)>	24.4	5.48
Completeness [%]	99.1	95.9
Redundancy	13.8	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	25% PEG4000, 100 mM MES, 0.2 M malic acid, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K