3DI2

Crystal structure of the complex of human interleukin-7 with unglycosylated human interleukin-7 receptor alpha ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-10
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	47.202, 113.021, 87.534
Unit cell angles	90.00, 105.10, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.212
Rwork	0.212
R-free	0.26700
RMSD bond length	0.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	21610
<I/σ(I)>	15.9	2.1
Completeness [%]	88.2
Redundancy	3.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	292	20% w/v PEG 8000, 0.1M MES, 0.2 M calcium acetate, pH 6.0, sitting drop, temperature 292K