3DDC
Crystal Structure of NORE1A in Complex with RAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.600, 88.000, 56.500 |
| Unit cell angles | 90.00, 125.00, 90.00 |
Refinement procedure
| Resolution | 19.590 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.590 | 1.850 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.086 | 0.348 |
| Number of reflections | 29894 | |
| <I/σ(I)> | 13.64 | 4.1 |
| Completeness [%] | 97.2 | 86.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100MM C2H3NAO2, 20% PEG 2000, 250MM (NH4)2SO4, 10MM DTE, pH 4.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
