3DCJ
Crystal structure of glycinamide formyltransferase (PurN) from Mycobacterium tuberculosis in complex with 5-methyl-5,6,7,8-tetrahydrofolic acid derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.153, 61.660, 61.525 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 32.580 - 2.200 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.24286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1meo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.859 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.580 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 22471 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 3350, 0.2 M Mg nitrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
