3D9O
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-09-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 |
Unit cell lengths | 57.444, 57.444, 107.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.760 - 2.000 |
R-factor | 0.20613 |
Rwork | 0.204 |
R-free | 0.23994 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3d9j |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 23561 | |
<I/σ(I)> | 17.4 | 4.3 |
Completeness [%] | 98.1 | 95.8 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.2M Li2SO4, 1.6M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |